FUS mutations and DNA damage

22 January 2014

Dr Majid Hafezparast and Professor Keith Caldecott have published a paper on DNA damage related to FUS mutations in Nucleic Acids Res. 2013 Sep 18, titled:

PARP-1 dependent recruitment of the amyotrophic lateral sclerosis-associated protein FUS/TLS to sites of oxidative DNA damage. 

They examined the possibility that FUS is also a component of the cellular response to DNA damage and showed that both GFP-tagged and endogenous FUS re-localize to sites of oxidative DNA damage induced by UVA laser, and that FUS recruitment is greatly reduced or ablated by an inhibitor of poly (ADP-ribose) polymerase activity.

GFP-FUS(R521G), harbouring a mutation that is associated with ALS, exhibited reduced ability to accumulate at sites of UVA laser-induced DNA damage. Together, these data suggest that FUS is a component of the cellular response to DNA damage, and that defects in this response may contribute to ALS.